Composite

Part:BBa_K429000:Design

Designed by: Stefano Fenu   Group: iGEM10_Duke   (2010-10-26)

Promoter Testing Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1572


Design Notes

Note: The assembly and transformation of the part can initially be tested by the induction of GFP expression in the presence of tetracycline or anhydrotetracycline.

Initial transformation of the part was successful; GC5 colonies carrying the part in the pSB1A3 plasmid fluoresced when excited by 500nm wavelength light.

BBa_J23100 was attached to K42900 by standard assembly; the part was then transformed into GC5 and plated on ampercilin agar. The colonies did not express GFP constitutively, but began to express GFP upon induction by aTc.

Comparable results were exhibited from the replacement of BBa_J23100 with a modified cI-lamda promoter that housed an AP-1 binding site.

Compatibility Information:

Chassis: Shown to work in GC5

Plasmids: Shown to be functional in pSB1A3, pSB1C3

Promoters: J23100 was successfully appended to the part by standard assembly; A modified cI lam promoter with recognition sites for AP-1 binding was successfully appended to the part by PCA;

Source

This is a composite of BBa_Q04400, and BBa_I13504.

References